Iran "will not accept any amendments in this agreement, be it now or in the future, and it will not allow any other issues to be linked to the JCPOA", the foreign ministry said in a statement, using the 2015 deal's technical name.
In the wake of anti-government protests in Iran, the Trump administration is also imposing targeted sanctions against several government officials for human rights abuses.
Trump is also waiving other USA penalties covered by the agreement, including on Iran's oil and gas sectors, which were up for renewal next week.
The action is the third time Mr Trump has given a reprieve to the agreement brokered by the Obama administration, despite having called it "one of the worst" agreements the U.S. has ever entered into.
The new sanctions come on top of previous designations announced back in May and October.
The nuclear pact was earlier struck in 2015 between Tehran and the six major powers to lift nuclear-related sanctions on the Islamic Republic in exchange for restrictions on its nuclear activities, paving the way for global business deals.
Following Trump's statement, Iranian Foreign Minister Javad Zarif said on Friday that the Joint Comprehensive Plan of Action (JCPOA) was not "renegotiable".
US President Donald Trump said on Friday he will extend sanctions relief on Iran under the 2015 nuclear deal for the last time, threatening a US withdrawal from the landmark pact unless US Congress and European allies can fix the alleged "disastrous flaws".
"The American administration has failed to undermine the nuclear deal ..."
This would not entail negotiations with Iran but would be the result of talks with European allies, the official said.
Earlier on Friday, Kremlin spokesman Dimitry Peskov stated regarding the deal that, "You know the position of the Russian side: we adhere to and are committed to the idea of the need to preserve this agreement on the Iranian nuclear dossier..."
United Nations inspectors have certified Iran's compliance with the deal nine times, most recently in November.
In conjunction with the waivers, the Treasury Department placed sanctions on 14 people and entities for alleged offenses unrelated to Iran's nuclear industry.
Zarif took to Twitter after the Brussels meeting to warn that "Iran's continued compliance (is) conditioned on full compliance by the U.S.".
Trump has reportedly complained behind the scenes that the deal makes the U.S. look weak.
Mr Trump also wants Iran's ballistic missile programme to be addressed.
Europeans say these issues should be kept separate from discussion of the deal, but in a nod to USA concerns, Mogherini stressed they were raised with Zarif on Thursday.
Rep. Eliot Engel, D-N.Y., the ranking Democrat on the House Foreign Relations Committee, said Trump's policy "sets impossible standards that would ultimately isolate the United States rather than isolating the regime in Tehran".
The sanctions with the most political repercussions are against the administrative head of Iran's judiciary, Sadegh Amoli Larijani.
Nordic American Tanker Shipping Ltd (NYSE: NAT ) has an ABR of 3.57 which is the combined stock view of 7 analysts poll results. A total of 2.08 Million shares exchanged hands, whereas the company's average trading volume stands at 1.9 Million shares.
However, they found themselves having to contain Kane , Son , Dele Alli and Christian Eriksen as Spurs' attacking four shone. Kane also became Spurs' all-time leading scorer in the Premier League , with his brace taking up to 98 league goals.
The Portuguese responded by alluding to Conte's hair transplant: "I'm not going to lose my hair to speak about Antonio Conte ". It was Alan Pardew's first league victory since he took over as manager nine matches ago at the start of December.
Emergency personnel evacuate local residents and their dogs through floodwaters January 9 after a mudslide in Montecito , Calif. His father, Pinit Sutthithepa, and 2-year-old sister, Lydia, are among the missing, the county sheriff's office said.
After the match, the 48-year-old said his players were exhausted after Wednesday's 0-0 draw with Arsenal in the Carabao Cup. The top-class goalkeeper has looked set to extend his contract in west London.
Per l'associazione "rincari del 13,91%, come per la Milano Serravalle , vuol dire mandare in tilt i bilanci dei pendolari". La Brescia-Bergamo-Milano ad esempio aumenta la tariffa autostradale del 4,69%.
He mentioned that members of the Dawa Party are free to support his State of Law Coalition or Abadi's Victory for Iraq Alliance. The alliance would aim to unify the country after a recent victory over "Islamic State" (IS) militants.
The comments came during a meeting over immigration reform, a topic that Democrats are trying to tie to the shutdown fight. Tom Cotton and David Perdue , said they "do not recall the President saying these comments specifically".
Jose Mourinho has suggested that Henrikh Mkhitaryan could be sold if Manchester United receive the right price for his services. The Armenian shone for United last season, when given the opportunity, finishing the campaign with 11 goals.
Trump's derogatory remarks cited by the Washington Post and New York Times were in reference to African countries and Haiti. Ghana is widely seen as a stable, peaceful country in an often turbulent region, and has close ties to the United States.
Pacers rally from 22-point deficit to beat Cavaliers 97-95
Overcoming a 20-0 run is rarely a recipe for success , but the Indiana Pacers had the right chefs in the kitchen on Friday night. The Cavaliers have dropped six of eight dating to a Christmas Day loss at defending champions the Golden State Warriors .
EPS Predictions Review: Tetra Tech, Inc. (TTEK)
The stock's 50-day average volume has been 1,228,184 shares, with an average of 25% of medium term indicators pointing toward Buy. A number of other hedge funds and other institutional investors have also added to or reduced their stakes in the business.
Magic Johnson tweets support for Luke Walton
This is despite the team trying to keep their relationship with the outspoken parent respectful as recently as last month. The Lakers ended their nine-game skid by defeating Atlanta 132-113. "So, it definitely felt good tonight".
Ant McPartlin announces divorce from wife Lisa
Ant's romance has been the talk of tabloids over the past few months ever since he entered rehab for an addiction to painkillers. And his health was reportedly a factor in Ant's decision to divorce Lisa, whom he has been with for 23 years and married in 2006.
Centurion Test: South Africa all out for 335
To repeat what I started with - I surely think this team is capable of making a comeback in the series. Ashwin got one to hold its line just enough after pitching to take the edge of Markram's bat.
State releases school graduation rates
"As long as we can stay within the mid 90s, as far our graduation rate is concerned, I'm going to be pretty excited about it". IN requires students to pass state tests to graduate unless they can get a waiver by meeting other criteria.
Lebanon: Hamas official's vehicle targeted
Images posted on social media and used by Lebanese TV show a mangled vehicle , a large fire and black smoke rising above the city. Sidon Mayor Mohammed Saudi told Al-Manar TV that one person was wounded in the explosion as he opened the auto door.